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dna plasmid pcdna3 flag pten  (Addgene inc)


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    Addgene inc dna plasmid pcdna3 flag pten
    Dna Plasmid Pcdna3 Flag Pten, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+flag+pten+plasmid/pcDNA3-FLAG+PTEN+(Plasmid+%2378777)/pmc11598603-65-46-50
    Average 93 stars, based on 12 article reviews
    dna plasmid pcdna3 flag pten - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: LKB1 deficiency promotes proliferation and invasion of glioblastoma through activation of mTOR and focal adhesion kinase signaling pathways
    Article Snippet: .. The pcDNA3-Flag PTEN plasmid (Addgene plasmid # 78777 [ 25 ] or control pcDNA3 plasmid (4.5 μg) with control or LKB1 siRNA (4.5 μl) was transfected into T98 cells seeded in 6 well-plates using Lipofectamin 2000 reagent. .. At 72 hours post-transfection, cell numbers were counted, and Flag-PTEN expression was measured by Western blot using anti-FLAG antibodies (Flag M2 antibody, Sigma, Saint Louis, MO).

    Article Title: Splicing factor SRSF1 controls T cell hyperactivity and systemic autoimmunity
    Article Snippet: The pcDNA3.1-SRSF1 expression plasmid was a gift from James Manley (Columbia University, NY), and the pGL3-PTEN 3′ UTR plasmid was a gift from Jeffrey Rosen (Addgene plasmid 28104). .. The pcDNA3-FLAG PTEN plasmid was a gift from Jaewhan Song (Addgene plasmid 78777). ..

    Article Title: Splicing factor SRSF1 controls T cell hyperactivity and systemic autoimmunity
    Article Snippet: The pcDNA3.1-SRSF1 expression plasmid was a gift from James Manley (Columbia University, NY), and the pGL3PTEN 3′ UTR plasmid was a gift from Jeffrey Rosen (Addgene plasmid 28104). .. The pcDNA3-FLAG PTEN plasmid was a gift from Jaewhan Song (Addgene plasmid 78777). ..

    Control:

    Article Title: LKB1 deficiency promotes proliferation and invasion of glioblastoma through activation of mTOR and focal adhesion kinase signaling pathways
    Article Snippet: .. The pcDNA3-Flag PTEN plasmid (Addgene plasmid # 78777 [ 25 ] or control pcDNA3 plasmid (4.5 μg) with control or LKB1 siRNA (4.5 μl) was transfected into T98 cells seeded in 6 well-plates using Lipofectamin 2000 reagent. .. At 72 hours post-transfection, cell numbers were counted, and Flag-PTEN expression was measured by Western blot using anti-FLAG antibodies (Flag M2 antibody, Sigma, Saint Louis, MO).

    Transfection:

    Article Title: LKB1 deficiency promotes proliferation and invasion of glioblastoma through activation of mTOR and focal adhesion kinase signaling pathways
    Article Snippet: .. The pcDNA3-Flag PTEN plasmid (Addgene plasmid # 78777 [ 25 ] or control pcDNA3 plasmid (4.5 μg) with control or LKB1 siRNA (4.5 μl) was transfected into T98 cells seeded in 6 well-plates using Lipofectamin 2000 reagent. .. At 72 hours post-transfection, cell numbers were counted, and Flag-PTEN expression was measured by Western blot using anti-FLAG antibodies (Flag M2 antibody, Sigma, Saint Louis, MO).



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